EMODnet Product Catalogue
Microlitter particles in water surface sampled with ferrybox in Danish waters autumn 2020
Microlitter was sampled with a module fitted on a Ferry Box system on the commercial ferry cruises (M/S Color Line Fantasy) between Oslo and Kiel. The system acquired samples in the Danish part of the Skagerrak and Kattegat. In total seven samples were collected from 23-10-2020 to 02-11-2020 using filters of mesh sizes of 300 and 500 µm. Particles were visually identified and analysed for chemical composition using FTIR. Particle concentrations ranged from 0.39 to 1.85 particles per m3 (average 0.91 per m3).
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| Date | 2021-10-08 |
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| Date type | Creation: Date identifies when the resource was brought into existence |
| Date type | Revision: Date identifies when the resource was examined or re-examined and improved or amended |
| Date | 2022-10-27 |
| Date type | Publication: Date identifies when the resource was issued |
| Date type | Publication: Date identifies when the resource was issued |
| Unique resource identifier | 3c122742-3516-4bde-8128-deeea6ebf3d0 |
Point of contact
| Organisation name | The Danish Environmental Protection Agency |
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| Delivery point | Haraldsgade 53 |
| City | Copenhagen Ø |
| Postal code | 2100 |
| Country | Denmark |
| Electronic mail address | mst@mst.dk |
| Linkage | https://eng.mst.dk/ |
| Role | Dataset Holding Organisation: Dataset Holding Organisation |
Point of contact
| Organisation name | Aarhus University, Department of Bioscience, Marine Ecology Roskilde |
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| Delivery point | 399 Frederiksborgvej P.O. Box 358 |
| City | Roskilde |
| Postal code | DK-4000 |
| Country | Denmark |
| Linkage | http://bios.au.dk/en/ |
| Role | Author: Party who authored the resource |
Descriptive keywords
| GEMET - INSPIRE themes, version 1.0 | |
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| SeaVoX salt and fresh water body gazetteer |
Storebaelt
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Kattegat
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| SeaDataNet Agreed Parameter Groups | Pollution |
| SeaDataNet Parameter Discovery Vocabulary |
Suspended particulate material grain size parameters
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Micro-litter in water bodies
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| Language | English: English |
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| Character set | UTF8: 8-bit variable size UCS Transfer Format, based on ISO/IEC 10646 |
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| Supplemental Information | Project report |
Reference System Information
| Anchor | WGS 1984 |
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| Hierarchy level | Dataset: Information applies to the dataset |
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| Statement | All the samples contained low levels of SPM and organic matter, and a bottle with prefiltered (filtered through a filter with pore size 0.22 µm) reverse osmosis (RO) water was used to rinse the sample material directly from the mesh filters to Whatman glass microfiber filters (grade GF/A, pore size 1.6 µm, Ø 47 mm). Filtration was carried out in sterile conditions in a laminar airflow cabinet (with HEPA filter) using a Nalgene vacuum filtration system. In most cases, the material on the mesh filters were filtered on to 1-3 GF/A filters depending on the amount of SPM. After filtration, the filters with material were immediately transferred to a petri dish and covered prior to drying and analysis. After filtration, all samples were analysed by visual identification followed by chemical confirmation of the polymer material. A Nikon SMZ745T stereo microscope (x20 magnification) with analysis software (Infinity Analyze v.6.5.6) was used to photograph and measure individual particles. The selection of particles was made following standard NIVA protocols which are mirrored in peer-reviewed literature (Lusher et al. 2020). Visual analysis followed where potential plastics were isolated, photographed, described in terms of morphology and color, and measured along the longest length (µm). The following definitions were used for fragments, fibers and bead according to GESAM (2019). Fragments are defined as irregular NIVA 7601-2021 11 shaped hard particles having appearance of being broken down from a larger piece of litter, fibers as long fibrous material that has a length substantially longer than its width and beads as hard particle with spherical, smooth or granular shape. All particles from each sample were subjected to further chemical characterization using µFTIR micro-scope analysis. This was performed on a Perkin Elmer Spotlight 400 µFTIR spectrometer. To improve the quality of the spectra generated, particles were prepared for analysis using a diamond compression cell (DCC) accessory. Particles were carefully transferred from glass microfiber filter papers to the DCC with use of extra fine micro-forceps. The DCC compresses the particles to a thin, homogeneous thickness. The DCC was then loaded onto the µFTIR microscope stage for analysis. Measurements were obtained in transmission mode and at 4 cm-1 spectral resolution for the range 4000 to 600 cm-1. Spectra were produced from a composite of 2 co scans. Background measurements were taken before each batch of particles was analysed. Library matching was performed in the Spectrum 10 software (v. 10.6.2). Each spectrum was compared to several different libraries available at NIVA: Perkin Elmer ATR Polymers library, ST Japan Polymers ATR library, BASEMAN library (Primpke et al. 2018), and several in-house libraries including reference polymers, different textile materials, and potential sources of laboratory contamination. All spectra were manually inspected to ensure that the library matches were acceptable. If the polymer type of a particle could not be confirmed (low intensity peaks, small particle size) but the spectra showed characteristic peaks of synthetic plastic it was included as ‘other plastic’. Field blank samples were performed on the vessel alongside sample collection. Field blanks consisted of the same filter material left exposed to air for the duration at which the filters were not housed inthe Ferrybox (during changing and packaging of the samples). The field blanks were performed to monitor for potential contamination during the changing of the filters. The field blanks were processed in the same way as the samples to represent any contamination introduced during the sampling procedure. One field blank was performed per sample, apart from CF2 where no field blank was per-formed. Three laboratory blanks were included in each round of filtration to test for laboratory contamination. Prefiltered RO-water was filtered at the start, in the middle and in the end of the filtration of the samples using the same set up as the samples. The purpose of this is to have control over the potential contamination during the whole filtration process with the blanks representing the levels of contamination that could have been introduced to the samples that were filtered the same day. |
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| File identifier | 3c122742-3516-4bde-8128-deeea6ebf3d0 | ||||||||||||||
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| Metadata language | English | ||||||||||||||
| Character set | UTF8: 8-bit variable size UCS Transfer Format, based on ISO/IEC 10646 | ||||||||||||||
| Hierarchy level | Dataset: Information applies to the dataset | ||||||||||||||
| Date stamp | 2025-01-28T00:00:00 | ||||||||||||||
| Metadata standard name | ISO 19115:2003/19139 | ||||||||||||||
| Metadata standard version | 1.0 | ||||||||||||||
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