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            <gco:CharacterString>10 sediment samples from the North Sea consisting of ~50 ml (~100 g wet weight (ww)) were first chemically digested by two hours of thoroughly shaking with 100 ml of a solution (consisting of ~3 % potassium hydroxide, ~1 % potassium tripolyphosphate, ~1 % potassium silicate and ~7 % sodium hypochlorite, so dominant fractions of natural organic matter were decomposed. Similar treatment of different types of common plastic materials showed no larger external changes in the particle structure. This was followed density, separation by adding 100 ml of saturated saline solution, followed by ten minutes of shaking and consequently five minutes of ultrasonic treatment. The particles in the samples were allowed to settle for five minutes after each of the two steps. Pooled supernatant was filtered into six size fractions, i.e. 20-38, 38-100, 100-300, 300-1000 and 1000-5000 µm, by being flushed with filtered freshwater through the sediment test sieves (Retsch) placed on top of each according to decreasing mesh size. For each size fraction, particles retained on the test sieves and regarded as of synthetic origin were visually identified as microplastic-like particles based on their relatively homogenous texture and structure using a stereo microscope (20-50x magnification). In size fraction 20-38 mm and 38 -100 µm only fibres longer than &gt;100 µm were counted and these data are in the data sheet aggregated with data for the size fraction 100 – 300 µm. For quality assurance of the analyses, all glassware was cleaned and baked in the oven at 450 °C prior to the start of the microplastic analysis, and all the reagents were filtered using 20 µm mesh sizes. Additionally, the risk for airborne particulate contamination was controlled every day of sample analysis</gco:CharacterString>
          </gmd:statement>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
</gmd:MD_Metadata>

