Bigelow Laboratory for Ocean Sciences
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This dataset contains coccolithophore abundance data from surface underway samples collected during the JC039 cruise in the Atlantic between Falmouth, UK and Punta Arenas, Chile in October to December 2009. Samples were collected from the non-toxic water supply 3 times per day, approximately every 3 to 4 hours spaced between CTD stations. Coccolith and cell counts were collected on Millipore HA (nitrocellulose) filters which were rinsed with a potassium tetraborate buffer and frozen at 20 degrees C. Samples were then dried and mounted onto slides using Norland Optical Adhesive. These were then enumerated by birefringence microscopy. These data were collected by scientists from the Bigelow Laboratory for Ocean Sciences and contribute to the Atlantic Meridional Transect (AMT) project.
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This dataset contains coccolithophore abundance data from surface underway samples collected during the AMT15 cruise in the Atlantic between the United Kingdom and Cape Town, South Africa in September-October 2004. Surface samples were collected approximately every 3 to 6 hours from the non-toxic water supply. Microscope enumeration of coccolithophores and coccoliths was performed by filtering a 100 - 500 millilitre water sample through a Millipore HA filter, which was then rinsed with borate buffer, and frozen in a petri dish until counted. The filter was placed on a glass microscope slide, and a 60 degrees Celsius Canada Balsam was placed on top of the filter, followed by a cover slip. The clarified filter was examined for birefringent and plated coccolithophore counts, with an Olympus BH2 microscope equipped with polarisation optics. For statistical reasons, 200 coccoliths or cells were counted from each sample, when available. These data were collected for the Atlantic Meridional Transect (AMT) project by scientists from the Bigelow Laboratory for Ocean Sciences.
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This dataset contains coccolithophore abundance data from CTD bottles collected during the AMT16 cruise in the Atlantic between Cape Town, South Africa and the United Kingdom in May-June 2005. Bottle samples were collected from 6 light depths from the pre-dawn (between approximately 03:00 - 04:30 local time) and noon CTD casts. Microscope enumeration of coccolithophores and coccoliths was performed by filtering a 100 - 500 millilitre water sample through a Millipore HA filter, which was then rinsed with borate buffer, and frozen in a petri dish until counted. The filter was placed on a glass microscope slide, and a 60 degrees Celsius Canada Balsam was placed on top of the filter, followed by a cover slip. The clarified filter was examined for birefringent and plated coccolithophore counts, with an Olympus BH2 microscope equipped with polarization optics. For statistical reasons, 200 coccoliths or cells were counted from each sample, when available. These data were collected for the Atlantic Meridional Transect (AMT) project by scientists from the Bigelow Laboratory for Ocean Sciences.
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This dataset contains coccolithophore abundance data from CTD bottle samples collected during the JC053 cruise in the Atlantic between Southampton, UK and Punta Arenas, Chile in October-November 2010. Niskin samples were collected during the pre-dawn CTD (second cast of the day) from six light depths (1%, 7%, 14%, 33%, 55%, 97%). Samples for microscopic enumeration of coccolithophores and coccoliths were collected on Millipore HA (nitrocellulose) filters and rinsed with potassium tetraborate buffer, then frozen at 20 degC. Samples were then dried, mounted onto slides using Norland Optical Adhesive and enumerated using by birefringent microscopy. These data were collected by scientists from the Bigelow Laboratory for Ocean Sciences and contribute to the Atlantic Meridional Transect (AMT) project.
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This dataset contains coccolithophore abundance data from CTD bottle samples collected during the JC039 cruise in the Atlantic between Falmouth, UK and Punta Arenas, Chile in October-December 2009. Niskin samples were collected during the pre-dawn CTD (second cast of the day) from six light depths (1%, 7%, 14%, 33%, 55%, 97%) and from two deeper (down to 200 m) depths for coccolith enumeration. Coccolith and cell counts were collected on Millipore HA (nitrocellulose) filters which were rinsed with a potassium tetraborate buffer and frozen at 20 degC. Samples were then dried and mounted onto slides using Norland Optical Adhesive. These were then enumerated by birefringence microscopy (Balch et al. 2011). These data were collected by scientists from the Bigelow Laboratory for Ocean Sciences and contribute to the Atlantic Meridional Transect (AMT) project.
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This dataset contains coccolithophore abundance data from surface underway samples collected during the JC053 cruise in the Atlantic between Southampton, UK and Punta Arenas, Chile in October-November 2010. Samples were also collected 4 times per day from the non-toxic water supply. Samples for microscopic enumeration of coccolithophores and coccoliths were collected on Millipore HA (nitrocellulose) filters and rinsed with potassium tetraborate buffer, then frozen at 20 degC. Samples were then dried, mounted onto slides using Norland Optical Adhesive and enumerated using by birefringent microscopy. These data were collected by scientists from the Bigelow Laboratory for Ocean Sciences and contribute to the Atlantic Meridional Transect (AMT) project.
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This dataset contains coccolithophore abundance data from CTD bottle samples collected during the AMT15 cruise in the Atlantic between the United Kingdom and Cape Town, South Africa in September-October 2004. Bottle samples were collected from 6 light depths from the pre-dawn (between approximately 03:00 - 04:30 local time) and noon CTD casts. Microscope enumeration of coccolithophores and coccoliths was performed by filtering a 100 - 500 millilitre water sample through a Millipore HA filter, which was then rinsed with borate buffer, and frozen in a petri dish until counted. The filter was placed on a glass microscope slide, and a 60 degrees Celsius Canada Balsam was placed on top of the filter, followed by a cover slip. The clarified filter was examined for birefringent and plated coccolithophore counts, with an Olympus BH2 microscope equipped with polarisation optics. For statistical reasons, 200 coccoliths or cells were counted from each sample, when available. These data were collected for the Atlantic Meridional Transect (AMT) project by scientists from the Bigelow Laboratory for Ocean Sciences.